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KMID : 0384119860060020385
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Korean Journal of Clinical Pathology
1986 Volume.6 No. 2 p.385 ~ p.392
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Study of Quantitative Urine Culture by Flooding Technique and Bacterial culture of the Causative Agents of Urinary Tract Infection at Room Temperature
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Abstract
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The urinary tract infection is one of the most common disease and quantitative urine culture is the only certain method for the diagnosis of the urinary tract infection.
So a simple, inexpensive technique for performing quantitative urine culture by the flooding technique, which is performed on blood agar and MacConkey agar plates, is presented and compared with usual loop inoculation method. The results are as follows;
1. Among 500 urine specimens, only 107 specimens (21.4%) reveal significant bacteriuria.
2. Among 500 urine specimens, 165 specimens reveal one or more colonies by
the flooding technique and 158 specimens by the loop inoculation method.
3. In the 7 specimens which grow only by the flooding technique, all of them
show below 103 organisms per ml.
4. All 158 specimens containing more than 103 organisms per ml give identical
colony counts obtained from flooding technique and loop inoculation method.
5. The most common causative organism for significant bacteriuria is Escherichia
coli (66/107, 61.4%), followed by Klebsiella, Enterobacter, Staphylococcus
aureus, seudomonas, Enterococcus and Proteus.
Flooding technique of bacterial culture has been tested not only at 37C but also at room temperature (20´C) by the calculated bacterial suspensions which are frequently isolated from the urine specimens (Escherichia coli, Klebsiella, Enterobacter, Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus, Pseudomonas). The results are as follows;
1. Compared with loop inoculation controls at 37°C, identical results are obtained from 205 consecutive specimens among total 210 specimens at 37°C and 20°C by flooding technique.
2. ≪´hen the bacterial suspensions are cultured at room temperature, at least 24
hours are needed for proper colony counts by flooding technique.
3. The size and morphology of the colonies, which are cultured for at least 30
hours at room temperature ,are similar to those cultured for 24 hours at 37°C.
4. There is no bacteria that does not grow at room temperature.
These results strongly suggest that culture either initially or totally at room
temperature is a reliable method of quantitative bacterial culture. In general
practice, it is scarecely practicable to refrigerate postal specimens, and in hospital
when a specimen is obtained in the evening and not processed until the next
morning there is unnecessary delay. This simple and reliable method of urine
culture, the flooding technique on blood and MacConkey agar plate and culture at
room temperature, which could be conveniently carried out by a patient or medical
practitioner at the bedside would be a great advantage for preventing of the 4 proliferation of contaminant microorganisms and for adequate quantitative urine culture. And it may be conveniently used as the screening method in surveys such as at antenatal clinics and for the patients with chronic or recurrent urinary tract infection.
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